After regressing out the batch and cell cycle effects, these HVGs were used for dimension reduction and clustering analysis to identify candidate subpopulations. ![]() Pathway analysis showed that these HVGs are associated with functional characteristics of classic MSCs, such as inflammation mediated by chemokine and cytokine signaling, integrin signaling, and angiogenesis. GO enrichment analysis of highly variable genes (HVGs) obtained from WJMSCs revealed that these genes are significantly enriched in extracellular region with binding function, involved in developmental process, signal transduction, cell proliferation, etc. Meanwhile, we analyzed publicly available adipose-derived MSC (ADMSCs) scRNA-seq data and performed transcriptome comparison between WJMSCs and ADMSCs at the single-cell level. We also isolated CD142 + and CD142 − WJMSCs based on scRNA-seq data and compared their proliferation capacity and “wound healing” potential in vitro. We investigated the gene expression profile via single-cell RNA sequencing (scRNA-seq) of human primary Wharton’s jelly-derived MSCs (WJMSCs) cultured in vitro from three donors. ![]() The underlying molecular mechanisms that define MSC heterogeneity remain unclear. However, MSCs cultured in vitro exhibit functional heterogeneity. Mesenchymal stem/stromal cells (MSCs) are multipotent cells with a promising application potential in regenerative medicine and immunomodulation.
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